Abstract
Streptococcus mutans are bacteria that have important role in caries development. When caries lesion is developing, it
may invade to deeper tissues such as dental pulp and initiate cellular defenses such as neutrophil. Neutrophil is type of
leukocytes which are important in phagocytosis process. If neutrophil fails in phagocytosis, neutrophil will be lysis and
cause viability of neutrophil unprotected. Temulawak rhizome extract is predicted to increase viability of neutrophil
because it contains curcuminoids, volatile oil, saponin, flavonoid, and tannin. This research aimed of this research was
to determine the viability of neutrophil exposed by S.mutans and incubated in temulawak rhizomes extract 2.5%, 5%,
7.5%,10%, and 20%. Twenty four samples were divided into 6 groups, consisting of negative control, temulawak rhizome
extract 2.5%, 5%, 7.5%, 10%, and 20%. The viability of neutrophil was observed under an inverted microscope using
trypan blue staining. This study concluded that temulawak rhizome extract 2.5%, 5%, 7.5%, and 10% could increase
viability of neutrophil exposed by S.mutans. Temulawak rhizome extract 5% has the effective concentration to increase
viability of neutrophil.
Recommended Citation
Purnamasari, Ika Wahyu; Astuti, Pudji; and Ermawati, Tantin
(2014)
"Viabilitas neutrofil yangdiinkubasi dalamekstrakrimpangtemulawak(Curcuma xanthorrhiza ) dan dipapar dengan Streptococcus mutans (Viability of neutrophil incubated in temulawak rhizome extract (Curcuma xanthorrhiza) and exposed by Streptococcus mutans),"
Dentofasial: Vol. 13:
Iss.
3, Article 13.
DOI: 10.15562/jdmfs.v13i3.403
Available at:
https://scholarhub.unhas.ac.id/dentofasial/vol13/iss3/13
Pages
135-140
Rights
©2016Ika Wahyu Purnamasari, Pudji Astuti, Tantin Ermawati
DOI
10.15562/jdmfs.v13i3.403