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Abstract

Objective: This study evaluated the biological activities of a gel formulated from Pangasius sp. Gelatin (PG) combined with pomegranate peel (Granati pericarpium) extract (PPE) and investigated its potential to promote oral mucosal wound healing.
Materials and Methods: Gel formulations were prepared using PG and PPE at different concentrations (2,5% - 10%). In vitro assays included antibacterial activity against Staphylococcus aureus, antifungal activity against Candida albicans, antioxidant capacity (ABTS assay), cytotoxicity (MTT assay), fibroblast migration (scratch wound assay), and gene expression analysis (RT-qPCR).
Results: PPE-containing gel formulations exhibited antibacterial, antifungal, antioxidant properties, along with good cytocompatibility. The combined formulations improved fibroblast migration in the scratch wound assay. The expression of collagen I, collagen III, elastin, and fibronectin in several treatment groups. However, most changes in extracellular matrix-realted gene expression were not statistically significant.
Conclusion: The PG-PPE gel demonstrated favorable antimicrobial, antioxidant, and cytocompatible, properties and supported fibroblast migration, suggesting its potential as a candidate material for supporting oral wound healing. However, further in vivo and clinical studies are required to confirm its safety, effectiveness, and underlying biological mechanisms.

Pages

341-348

DOI

10.65844/2503-0825.1519

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