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Abstract

Objective: Given the role of Porphyromonas gingivalis in pulp inflammation and the potential of natural agents to enhance conventional medicaments, this study aimed to investigate the antibacterial activity of Moringa oleifera extract and chitosan, in combination with calcium hydroxide [Ca(OH)₂], against P. gingivalis

Material and Methods: The laboratory experiment used 12 groups with three replicates (36 samples in total). The groups included: (1) Moringa oleifera with Ca(OH)₂, (2) chitosan with Ca(OH)₂, and (3) M. oleifera, chitosan, and Ca(OH)₂ mixture. Each group had four subgroups: Ca(OH)₂ control and concentration ratios of 1:1, 1.5:1, and 2:1. Antibacterial activity was measured via disc diffusion at 24, 48, and 72 h, followed by One-Way ANOVA and Kruskal-Wallis test.

Results: M. oleifera combined with calcium hydroxide showed increased inhibition reaching 10.9 mm at 72 h (p = 0.137). Chitosan-calcium hydroxide peaked at 48 h (12.7 mm), decreasing by 72 h (p = 0.092). The diameter of the three-component mixture reached 11.0 mm at 72 h (p = 0.079).            

Conclusions: Although the combinations did not significantly enhance the antibacterial effect of calcium hydroxide, trends suggest a potential synergistic effect at higher concentrations and with prolonged exposure.

Keywords: Anti-bacterial agents, Calcium hydroxide, Chitosan, Moringa oleifera, Porphyromonas gingivalis

Pages

210-214

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

©2025Adilah ZF. Djerman, Muhammad F. Sabirin, Erna A. Sutanto, Nisrina E. Nasrun, Maria Tanumiharja

DOI

10.15562/jdmfs.v10i4.2110

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