Characterization and biocompatibility test of chitosan-alginate-fucoidan hydrogel as potential socket preservation material: In vitro study

Authors

Ainiyyah F. ZaizafunFollow
Nurlindah Hamrun, Department of Oral Biology, Faculty of Dentistry, Hasanuddin UniversityFollow
Erni Marlina, Department of Oral Medicine, Faculty of Dentistry, Hasanuddin UniversityFollow
Lenni Indriani, Department of Dental Material, Faculty of Dentistry, Hasanuddin UniversityFollow
Nurhayaty Natsir, Department of Conservative Dentistry, Faculty of Dentistry, Hasanuddin UniversityFollow
Asdar Gani, Department of Periodontology, Faculty of Dentistry, Hasanuddin UniversityFollow
Arifin, Department of Physics, Faculty of Mathematics and Natural Sciences, Hasanuddin UniversityFollow

Abstract

Objective: This study aimed to characterize and evaluate the in vitro biocompatibility of chitosan-alginate-fucoidan hydrogel at different concentrations as a potential socket preservation material.

Material and Methods: Hydrogels were prepared using various concentrations of chitosan-alginate-fucoidan formulas. Alginate and fucoidan which were extracted from brown algae, were dissolved in purified water with stirring, then mixed with chitosan from white shrimp skin, which was prepared in 2% acetic acid. The hydrogels were characterized using scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FTIR). Biocompatibility was assessed using KUSA cells through cell viability, cytotoxicity, and cell adhesion. Data were analyzed using a one-way ANOVA, with Tukey's post-hoc test for multiple comparisons. Statistical significance was defined as a p-value of ≤ 0.05.

Results: SEM analysis revealed distinct surface structures among scaffolds, with Formula 3 demonstrating a solid structure. The cell viability assay showed that all of the scaffolds had excellent cell viability with no significant statistical differences (p>0.05), with alginate-chitosan showing the highest viability. The cytotoxicity assay showed that all of the scaffolds were non-toxic; however, Formula-3 had a statistically significant higher level of cytotoxicity than Alginate-Fucoidan (p<0.05). However, it was still within safe limits. Chitosan-based formulations, including alginate-chitosan and Formulas 1-3, promoted superior cell attachment and morphological adaptation compared to pure alginate.

Conclusion: Chitosan-alginate-fucoidan hydrogels demonstrated good biocompatibility. Specifically, Formula-1 and Formula-2 achieved an optimal balance of high cell viability, effective cell adhesion, and low cytotoxicity. This combination of biomaterials exhibits no severe toxic effects and is compatible with the KUSA cell line.

Recommended Citation

Zaizafun, Ainiyyah F.; Hamrun, Nurlindah; Marlina, Erni; Indriani, Lenni; Natsir, Nurhayaty; Gani, Asdar; and Arifin (2025) "Characterization and biocompatibility test of chitosan-alginate-fucoidan hydrogel as potential socket preservation material: In vitro study," Journal of Dentomaxillofacial Science: Vol. 10: Iss. 4, Article 5.
DOI: https://doi.org/10.15562/jdmfs.v10i4.2096
Available at: https://scholarhub.unhas.ac.id/jdmfs/vol10/iss4/5

Pages

203-209

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

©2025Ainiyyah F. Zaizafun, Nurlindah Hamrun, Erni Marlina, Lenni Indriani, Nurhayaty Natsir, Asdar Gani, Arifin

DOI

10.15562/jdmfs.v10i4.2096