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Abstract

Objective: Tooth extraction is a common medical procedure that can be classified classified as simple or complex, depending on the condition of the tooth. Supportive therapies are being explored to  accelerate wound healing after tooth extraction. Hyaluronic Acid (HA) and Limosilactobacillus reuteri (L. reuteri) are known to have positive effects on overall body and oral health. This study aimed to analyze the differences in collagen density during wound healing process after tooth extraction with the administration of supportive therapy by combining HA and probiotic L. reuteri in Wistar rats (Rattus norvegicus).

Material and Methods: Collagen density was measured using Masson's trichrome staining. Observations were performed with light microscope at 400x magnification and Fiji/ImageJ software. Collagen measurement was observed on the blue-colored surface of the specimen. The unit of collagen density obtained in a single field of view was expressed as an area percentage (area%).

Result: One-way ANOVA showed significant differences in collagen density between the groups on days 3 (p = 0.005), 7 (p<0.001), and 14 (p= 0.009). In the combination therapy group (T3), the average collagen density was significantly higher than in the other groups on days 7 and 14 (p <0 .05).

Conclusion: Supportive therapy combining HA and L. reuteri probiotic combination therapy can increase collagen density during wound healing in Wistar rats, with the strongest effects observed on days 7 and 14.

Pages

194-197

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

©2025Desak MH. Wijayanti, Armelia S. Widyarman, Melanie H. Sadono, Firstine K. Hartanto, Komariah, Wiwiek Poedjiastuti

DOI

10.15562/jdmfs.v10i4.2024

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