Abstract
Objective: This study investigated the osteoinductive activity effects of freeze-dried homologous platelet-rich plasma (FD HPRP) by assessing its effects on type I collagen expression in MG-63 cells.
Material and Method: HPRP was obtained from a blood bank and subjected to freeze-drying and y-radiation (20 and 25 kGy) for sterilization. Osteoblasts (MG63 cells) were cultured to confluence and then divided into three groups. Group 1 was an untreated control, and groups 2 and 3 were treated with FD HPRP at 20 and 25 kGy, respectively. Type I collagen expression was examined using immunohistochemistry on day14.The data were analyzed by one-way analysis of variance.
Results: Collagen type 1 expression significantly differed between the FD HPRP and control groups (p < 0.05). Type I collagen expression was highest in the FD HPRP 25 group.
Conclusion: FD HPRP can increase collagen type 1 expression, supporting its potential to induce bone formation and suggesting future clinical applications.
Recommended Citation
Murdiastuti, Kwartarini and Olivia, Niken
(2026)
"Effects of freeze-dried homologous platelet-rich plasma on type I collagen expression in MG-63 cells: An in vitro study,"
Journal of Dentomaxillofacial Science: Vol. 11:
Iss.
1, Article 11.
DOI: https://doi.org/10.65844/2503-0825.1483
Available at:
https://scholarhub.unhas.ac.id/jdmfs/vol11/iss1/11
Pages
61
DOI
10.65844/2503-0825.1483