Abstract
Objective: This study investigated the osteoinductive activity effects of freeze-dried homologous platelet-rich plasma (FD HPRP) by assessing its effects on type I collagen expression in MG-63 cells.
Material and Method: HPRP was obtained from a blood bank and subjected to freeze-drying and y-radiation (20 and 25 kGy) for sterilization. Osteoblasts (MG63 cells) were cultured to confluence and then divided into three groups. Group 1 was an untreated control, and groups 2 and 3 were treated with FD HPRP at 20 and 25 kGy, respectively. Type I collagen expression was examined using immunohistochemistry on day14.The data were analyzed by one-way analysis of variance.
Results: Collagen type 1 expression significantly differed between the FD HPRP and control groups (p < 0.05). Type I collagen expression was highest in the FD HPRP 25 group.
Conclusion: FD HPRP can increase collagen type 1 expression, supporting its potential to induce bone formation and suggesting future clinical applications.
Recommended Citation
Murdiastuti, Kwartarini and Olivia, Niken
(2026)
"Oral hygiene behavior and its association with plaque, salivary flow rate, and veillonella parvula in Indonesian boarding school adolescents,"
Journal of Dentomaxillofacial Science: Vol. 11:
Iss.
1, Article 11.
DOI: https://doi.org/10.65844/2503-0825.1483
Available at:
https://scholarhub.unhas.ac.id/jdmfs/vol11/iss1/11
Pages
61
DOI
10.65844/2503-0825.1483