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Abstract

Objective: To assess the efficacy of irrigation solution containing reuterin in inhibiting gene expression of E. faecalis, F. nucleatum, and P. gingivalis during biofilm formation in ex vivo root canal model. Material and Methods: Reuterin was isolated from an Indonesian strain of L. reuteri. Seventy-two premolar root samples were subjected to inoculation with E. faecalis, F. nucleation, and P. gingivalis for 24 hours. Subsequently, the specimens underwent irrigation with a saline solution as negative control, 25% sodium hypochlorite (NaOCl) as positive control, 25 pg/ml of reuterin derived from a L. reuteri strain indigenous to Indonesia, and 25 pg/ml of L. reuteri Prodentis from BioGaia, Sweden. The number of colonies forming units (CFUs) in the irrigated samples were counted, and gene expression was detected by a real-time quantitative polymerase chain reaction (RT-qPCR) assay. Results: Reuterin effectively suppresses the growth of E. faecalis, F. nucleation, and P. gingivalis. Moreover, it notably reduced the transcription of genes (gelE, atlA, fadA, rade, fintA, and mfal) associated with biofilm formation (p<0.05). Conclusion: The reuterin extracted from L. reuteri hindered the development of biofilms by E. faecalis, F. nucleatum, and P. gingivalis. This inhibition occurred through the down-regulation of transcription in genes associated with biofilm formation.

Pages

88-94

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

©2024Armelia S. Widyarman, Muhammad I. Rizal, Thalianabilla Khalisha, Denisa N. Amanda, Deandra CN. Wiriawan

DOI

10.15562/jdmfs.v9i2.1702

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