Abstract
Pulp Out, a combination of root extract of sidaguri (Sida rhombifolia), the sap of jatropha (Jatropha curcas), and melittin, have been proven by in vivo study as a tooth-pulp devitalizing agent which has similar properties to arsenic, but has limited side effects. Pulp Out needs to be formulated in a stable dosage form to optimize the application. The goal of the present study was to produce a stable paste-containing Pulp Out using sodium carboxymethylcellulose (Na CMC, 1.25 (F1), 2.5 (F2), 5.0 (F3)) and hydroxypropyl methylcellulose (HPMC, 1.25 (F4), 2.5 (F5), 5.0 (F6)). The effectiveness of the paste was evaluated on the Vero cell line using an MTT assay. The results show that only F3 were in accordance with the requirements for good pharmaceutical properties (color, homogeneity, consistency, and presence of particles, pH and viscosity). The effectiveness test illustrated that F3 at a concentration of 6.25 µg/mL could inhibit the growth of the Vero cell line by 39.65 ± 3.05%, which was higher than melittin (31.40 ± 2.98%); the sap of jatropha (37.41 ± 1.11%); and root extract of sidaguri (25.22 ± 5.96%). In conclusion, Pulp Out can be formulated in paste using 5% of Na CMC, which is physically stable and effective as a tooth-pulp devitalizing agent.
Recommended Citation
Tanumihardja, Maria; Pakki, Ermina; Mandey, Fredryk; and Muslimin, Lukman
(2024)
"New formulation of devitalize paste containing Pulp Out for tooth application,"
Journal of Dentomaxillofacial Science: Vol. 9:
Iss.
3, Article 10.
DOI: https://doi.org/10.15562/jdmfs.v9i3.1759
Available at:
https://scholarhub.unhas.ac.id/jdmfs/vol9/iss3/10
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
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©2025Maria Tanumihardja, Ermina Pakki, Fredryk Mandey, Lukman Muslimin
DOI
10.15562/jdmfs.v9i3.1759