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Abstract

Objective: This study aimed to determine whether P. gingivalis induction in the gingival sulcus increased IL-6 expression by macrophages in the ovaries.

Material and Methods: This research was a laboratory experiment using a post-test-only control group design, using female white rats strain Sprague Dawley, six weeks old and in the estrous phase. They were divided into the control and treatment groups. The animal models were induced with P. gingivalis in the mesial gingival sulcus of their first right and left lower jaw molars, with 0.05 ml every three days for 19 days. On 0, 7, 14 days after bacteria induction, the animal models were euthanasia and removed their ovaries. The ovaries slides were stained by immunohistochemistry using IL-6 monoclonal antibody (diluted 1:100). The observation used light microscope in 10 fields of view with a magnification of 400x. Then, data were tested statistically with ANOVA (p<0.05).

Results: The expression of IL-6 in the macrophages of the ovaries of the female periodontitis model was higher than in the control group. However, only in the observation 14 days after the last induction, the expression of IL-6 in the ovaries of the periodontitis model group (12.86 ± 3.81) was significantly higher both with the control group (5.71 ± 2.76), with the female periodontitis model group on day 0 (6.86 ± 2.55), and day 7 after the last induction (8.71 ± 3.04).

Conclusion: P. gingivalis promoted increased expression of IL-6 in ovaries of female periodontitis models.

Pages

194-200

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Rights

©2024Agustin WS. Dharmayanti, Ernie M. Setiawatie, Hendy Hendarto

DOI

10.15562/jdmfs.v9i3.1825

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